Vacuum ultraviolet (VUV) absorption spectroscopy was recently introduced as a new detection system for just one, as well as comprehensive two-dimensional gas chromatography (GC×GC) and effectively applied to the analysis of various analytes in several matrices. In this scholarly study, its suitability for the analysis of breathing metabolites was looked into and the impact of a finite level of the absorption cell and makeup gas pressure was examined for volatile analytes in terms of awareness and chromatographic quality. A commercial available VUV absorption spectrometer was coupled to GC×GC and applied to the evaluation of highly polar volatile organic compounds (VOCs). Breath-gas samples were acquired by needle snare micro extraction (NTME) throughout a glucose challenge and analyzed by the applied technique.
Regarding qualitative and quantitative information, the VGA-100 is compatible with common GC×GC recognition systems like FID and even TOFMS. Average maximum widths of 300 LODs and is in the lower and range were achieved using GC×GC-VUV. Especially small-oxygenated breath metabolites show extreme and characteristic absorption patterns in the VUV region. Challenge responsive VOCs could be monitored and determined during a glucose challenge. The new VUV recognition technology might be of great benefit for applications in scientific research especially.
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